Skip Navigation



Alcohol and Alcoholism Advance Access published online on March 22, 2006
Alcohol and Alcoholism, doi:10.1093/alcalc/agl009
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
41/3/240    most recent
agl009v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by BEST, C. A.
Right arrow Articles by SZCZEPIORKOWSKI, Z. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by BEST, C. A.
Right arrow Articles by SZCZEPIORKOWSKI, Z. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the Medical Council on Alcohol. All rights reserved
Received May 31, 2005
Revised January 21, 2006
Accepted January 23, 2006

Article

METHOD TO ASSESS FATTY ACID ETHYL ESTER BINDING TO ALBUMIN

CATHERINE A. BEST 1 *, MICHAEL LAPOSATA 2, VERONIA G. PROIOS 1, and ZBIGNIEW M. SZCZEPIORKOWSKI 3

1 Department of Biomedical Sciences, Northeastern University, Boston, MA 02115, USA; Division of Laboratory Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
2 Division of Laboratory Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
3 Division of Laboratory Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Department of Pathology, Dartmouth-Hitchcock Medical Center and Dartmouth Medical School, Lebanon, NH 03756, USA

* To whom correspondence should be addressed.
CATHERINE A. BEST, E-mail: cbest1{at}partners.org


  Abstract

Aims: To develop a method to assess the relative binding of fatty acid ethyl esters (FAEE) and free fatty acids (FFA) to albumin, and to determine if binding affinity is related to fatty acid chain length and or degree of saturation. Methods: Radiolabelled ethyl-[14C] oleate -bound to albumin was challenged with various ratios of FFA to FAEE. The displacement of ethyl-[14C] oleate by FFA was visualized and quantitated through a combination of native-PAGE, autoradiography, and liquid scintillation counting (LSC). Results: As the ratio of FFA to FAEE increased from 0:1 to 12:1, for all fatty acids tested (myristate, palmitate, stearate, oleate, linoleate, and arachidonate), ethyl-[14C] oleate displacement increased, when expressed as radioactivity (in DPM) as a percentage of control. In contrast, ethyl oleate did not displace stearate or oleate from albumin at molar ratios up to 5:1 (FAEE:FFA). Conclusions: The method developed gave reproducible visualization of noncovalent binding of radiolabelled FAEE to albumin. The combination of native-PAGE and autoradiography LSC works well in assessing the binding properties of albumin and radiolabelled FAEE. The data indicate preferential binding of FFA over FAEE to albumin with six different FFA displacing FAEE to an approximately equal extent.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.