Skip Navigation



Alcohol and Alcoholism Advance Access published online on August 30, 2005
Alcohol and Alcoholism, doi:10.1093/alcalc/agh196
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
40/6/485    most recent
agh196v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by PATEL, V. B.
Right arrow Articles by PREEDY, V. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by PATEL, V. B.
Right arrow Articles by PREEDY, V. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the Medical Council on Alcohol. All rights reserved
Received March 2, 2005
Revised July 15, 2005
Accepted July 26, 2005

Article

PROTEIN ADDUCT SPECIES IN MUSCLE AND LIVER OF RATS FOLLOWING ACUTE ETHANOL ADMINISTRATION

VINOOD B. PATEL 1*, SIMON WORRALL 2 {dagger}, PETER W. EMERY 3, and VICTOR R. PREEDY 3

1 Department of Biomedical Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK
2 Alcohol Research Unit, Department of Biochemistry and Molecular Biology, The University of Queensland, Brisbane, Queensland, QLD 4072, Australia
3 Nutrition, Food and Health Research Centre, King's College London, 150 Stamford Street, London SE1 9NN, UK

* To whom correspondence should be addressed.
VINOOD B. PATEL, E-mail: v.b.patel{at}westminster.ac.uk


  Abstract

Aims: Previous immunohistochemical studies have shown that the post-translational formation of aldehyde-protein adducts may be an important process in the aetiology of alcohol-induced muscle disease. However, other studies have shown that in a variety of tissues, alcohol induces the formation of various other adduct species, including hybrid acetaldehyde-malondialdehyde-protein adducts and adducts with free radicals themselves, e.g. hydroxyethyl radical (HER)-protein adducts. Furthermore, acetaldehyde-protein adducts may be formed in reducing or non-reducing environments resulting in distinct molecular entities, each with unique features of stability and immunogenicity. Some in vitro studies have also suggested that unreduced adducts may be converted to reduced adducts in situ. Our objective was to test the hypothesis that in muscle a variety of different adduct species are formed after acute alcohol exposure and that unreduced adducts predominate. Methods: Rabbit polyclonal antibodies were raised against unreduced and reduced aldehydes and the HER-protein adducts. These were used to assay different adduct species in soleus (type I fibre-predominant) and plantaris (type II fibre-predominant) muscles and liver in four groups of rats administered acutely with either [A] saline (control); [B] cyanamide (an aldehyde dehydrogenase inhibitor); [C] ethanol; [D] cyanamide+ethanol. Results: Amounts of unreduced acetaldehyde and malondialdehyde adducts were increased in both muscles of alcohol-dosed rats. However there was no increase in the amounts of reduced acetaldehyde adducts, as detected by both the rabbit polyclonal antibody and the RT1.1 mouse monoclonal antibody. Furthermore, there was no detectable increase in malondialdehyde-acetaldehyde and HER-protein adducts. Similar results were obtained in the liver. Conclusions: Adducts formed in skeletal muscle and liver of rats exposed acutely to ethanol are mainly unreduced acetaldehyde and malondialdehyde species.

{dagger}Author to whom reprint requests should be addressed at: Alcohol Research Unit, Department of Biochemistry and Molecular Biology, The University of Queensland, Brisbane, Queensland, QLD 4072, Australia. Tel: +61 7 3365 4626; Fax: +61 7 3365 4699; E-mail: s.worrall@uq.edu.au
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.