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Alcohol and Alcoholism Advance Access published online on October 11, 2004
Alcohol and Alcoholism, doi:10.1093/alcalc/agh103
© 2004 by Medical Council on Alcohol
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Received September 1, 2004
Revised September 19, 2004
Accepted September 19, 2004

Article

ORAL CHRONIC ETHANOL ADMINISTRATION TO RODENTS BY AGAR GEL DIET

I. BYKOV 1, M. PALMÉN 1, L. PIIRAINEN 1, and K. O. LINDROS 1*

1 National Public Health Institute, Department of Mental Health and Alcohol Research, POB 33, 00251 Helsinki, Finland

* To whom correspondence should be addressed. E-mail: kai.lindros{at}ktl.fi.


  Abstract

Background: Chronic ethanol administration to rodents requires specially designed equipment and is labor intensive. Here we report a new procedure. Method: A commercial liquid diet preparation was made into a gel by addition of 0.5% agar. The gel, containing 5.3% ethanol, was offered in Falcon tubes equipped with a feeding opening. Results: The gel consumption by C57/Bl mice resulted in high blood ethanol levels (average 43 mM). After 6 weeks, marked liver steatosis and significantly increased serum alanine aminotransferase levels had developed. Conclusions: Administration of ethanol in a nutritionally adequate gel provides a simple method for studies on chronic ethanol effects in rodents.


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