Alcohol and Alcoholism Advance Access published online on August 10, 2004
Alcohol and Alcoholism, doi:10.1093/alcalc/agh085
© 2004 by Medical Council on Alcohol
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1 Department of Pharmacology, Physiology and Therapeutics, University of North Dakota School of Medicine and Health Science, Grand Forks, ND 58203, USA
* To whom correspondence should be addressed. E-mail: jren{at}uwyo.edu.
Aims: Chronic alcohol exposure leads to a deficiency of group B vitamins and increased risk of alcoholic cardiomyopathy characterized by impaired ventricular contractility. This study was designed to examine the effect of group B vitamin supplementation on short-term exposure of the main alcohol metabolite acetaldehyde (ACA)-induced cardiac contractile dysfunction in rat ventricular myocytes. Methods: Mechanical contractile properties were evaluated by an IonOptix SoftEdge® system. Protein damage and apoptosis were determined by protein carbonyl and caspase-3 assays, respectively. Results: Short-term (4-6 h) culture of myocytes with ACA (10 µM) depressed peak shortening amplitude, maximal velocity of shortening/relengthening, shortened duration of shortening but not the duration of relengthening. ACA exposure also enhanced protein carbonyl formation and apoptosis in ventricular myocytes. The toxin-induced mechanical defects, protein damage and apoptosis were ablated by vitamin B1 (10 µM), an essential vitamin required for DNA synthesis and repair. Vitamin B6 (10 µM) attenuated ACA-induced impairment of shortening duration. Vitamin B12 (1 mM) attenuated ACA-induced reduction in maximal velocity of shortening/relengthening. Unlike vitamin B1, none of the other ACA-elicited alterations in myocyte mechanical function were affected by vitamin B6 or vitamin B12. Vitamin B6 and vitamin B12 partially, but significantly, attenuated the ACA-induced carbonyl formation without affecting ACA-induced apoptosis. Conclusions: These data provide evidence that vitamin B1 supplementation may be protective for ACA-induced cytotoxicity through protection against protein damage and apoptotic cell death in ventricular myocytes.
Revised May 12, 2004
Accepted June 25, 2004
Article
ACETALDEHYDE-INDUCED CARDIAC CONTRACTILE DYSFUNCTION MAY BE ALLEVIATED BY VITAMIN B1 BUT NOT BY VITAMINS B6 OR B12
2 North Dakota Fetal Alcohol Syndrome Center, University of North Dakota School of Medicine and Health Science, Grand Forks, ND 58203, USA
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