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Alcohol and Alcoholism Vol. 37, No. 4, pp. 327-329, 2002
© 2002 Medical Council on Alcohol

THE EFFECTS OF ETHANOL ON GLUCOSE 6-PHOSPHATE DEHYDROGENASE ENZYME ACTIVITY FROM HUMAN ERYTHROCYTES IN VITRO AND RAT ERYTHROCYTES IN VIVO

Mehmet Emin Büyükokuroglu, Sayit Altikat1 and Mehmet Çiftçi2

Departments of Pharmacology and
1 Biochemistry, Medical Faculty and
2 Biotechnology Application and Research Center, Atatürk University, Erzurum, Turkey

Received 12 July 2001; first review notified 25 January 2002; accepted 13 February 2002

— Aims: The effects of ethanol on erythrocyte glucose 6-phosphate dehydrogenase (G6PD) activity were investigated under in vitro and in vivo conditions. Methods: For in vitro studies, glucose 6-phosphate dehydrogenase was purified from human erythrocyte and rats were used for in vivo studies. Enzyme activity was determined spectrophotometrically by the Beutler method. Results: The in vitro study showed that the I50 value was 17 mM for ethanol. In the case of the in vivo study, a 2 ml/kg dose of ethanol significantly inhibited the G6PD activity. The inhibition rate after ethanol administration was 59%, 40% and 6% at 1, 3 and 6 h after, respectively. Conclusions: The results of this study suggest that ethanol has a significant inhibitory effect on the G6PD activity both in vivo and in vitro.


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