Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Koch, O.
Right arrow Articles by Galeotti, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koch, O.
Right arrow Articles by Galeotti, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Alcohol and Alcoholism Vol. 35, No. 2, pp. 159-163, 2000
© 2000 Medical Council on Alcoholism

REGULATION OF MANGANESE SUPEROXIDE DISMUTASE (MnSOD) IN CHRONIC EXPERIMENTAL ALCOHOLISM: EFFECTS OF VITAMIN E-SUPPLEMENTED AND -DEFICIENT DIETS

Osvaldo Koch, Stella Farré, Maria Emilia De Leo1, Paola Palozza1, Bernardetta Palazzotti1, Silvia Borrelo1, Guglielmo Palombini1, Ameris Cravero and Tommaso Galeotti1,*

Departmento de Patología, Facultad de Medicina, Universidad de Buenos Aires, J. Uriburu 950, P.P. 1114 Buenos Aires, Argentina and
1 Istituto di Patologia Generale, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Rome, Italy

Received 3 June 1999; first review notified 6 October 1999; accepted 20 October 1999

In order to investigate the pathogenic mechanism responsible for liver injury associated with chronic alcoholism, we studied the effects of different dietary vitamin E levels in chronically ethanol (EtOH)-fed rats on the activity and mRNA regulation of the manganese superoxide dismutase (MnSOD) enzyme. Evidence is accumulating that intermediates of oxygen reduction may in fact be associated with the development of alcoholic liver disease. Since low vitamin E liver content seems to potentiate EtOH-linked oxidative stress, we studied the effect of EtOH treatment in livers from rats fed a diet deficient or supplemented with vitamin E. Chronic EtOH feeding enhanced hepatic consumption of vitamin E in both groups of EtOH-treated animals, irrespectively of the vitamin E level of the basal diet and the effect was observed in both the microsomal and mitochondrial fractions. Both EtOH-fed groups exhibited increased MnSOD gene expression, while the enzyme activity was enhanced only in the vitamin E-deprived group of EtOH-treated animals. The significant increase in manganese liver content found only in this last group could explain the rise of enzyme activity. In fact, in the absence of a parallel increase of the prosthetic ion manganese, MnSOD mRNA induction was not accompanied by a higher enzymatic activity. These findings support the role of oxidative alteration in the EtOH-induced chronic hepatotoxicity in which MnSOD response might represent a primary defence mechanism against the damaging effect of oxygen radical species.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 CarcinogenesisHome page
J.-Y. Choi, M. L. Neuhouser, M. J. Barnett, C.-C. Hong, A. R. Kristal, M. D. Thornquist, I. B. King, G. E. Goodman, and C. B. Ambrosone
Iron intake, oxidative stress-related genes (MnSOD and MPO) and prostate cancer risk in CARET cohort
Carcinogenesis, May 1, 2008; 29(5): 964 - 970.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
D. St. Clair
Manganese Superoxide Dismutase: Genetic Variation and Regulation
J. Nutr., November 1, 2004; 134(11): 3190S - 3191S.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. D. Wheeler, M. Nakagami, B. U. Bradford, T. Uesugi, R. P. Mason, H. D. Connor, A. Dikalova, M. Kadiiska, and R. G. Thurman
Overexpression of Manganese Superoxide Dismutase Prevents Alcohol-induced Liver Injury in the Rat
J. Biol. Chem., September 21, 2001; 276(39): 36664 - 36672.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.