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Alcohol and Alcoholism Advance Access originally published online on July 30, 2008
Alcohol and Alcoholism 2008 43(5):569-576; doi:10.1093/alcalc/agn058
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© The Author 2008. Published by Oxford University Press on behalf of the Medical Council on Alcohol. All rights reserved

Automated Measurement of Carbohydrate-Deficient Transferrin Using the Bio-Rad %CDT by the HPLC Test on a VariantTM HPLC System: Evaluation and Comparison with Other Routine Procedures

François Schellenberg1,*, Louise Mennetrey2, Catherine Girre3, Bertrand Nalpas4 and Jean Christophe Pagès1

1 Laboratoire de Biochimie et Biologie Moléculaire, Pôle de Biologie, Hôpital Trousseau, CHRU de Tours, France
2 CCAA, CHRU, Tours, France
3 Addictology Unit, Hôpital Fernand Widal, Paris, France
4 INSERM U567, Hôpital Necker, Paris, France

* Corresponding author: Laboratoire de Biochimie et Biologie Moléculaire, Pôle de Biologie, Hôpital Trouseau, 37044 Tours, France. Tel: +33-2-47474684; Fax: +33-2-47474688; E-mail: f.schellenberg{at}chu-tours.fr

Received 9 November 2007; first review notified 3 January 2008; in revised form 22 May 2008; accepted 24 June 2008


  Abstract

Aims: In this study, we evaluated the new %CDT by the HPLC method (Bio-Rad, Germany) on a VariantTM HPLC system (Bio-Rad), checked the correlation with well-known methods and calculated the diagnostic value of the test. Methods: Intra-run and day-to-day precision values were calculated for samples with extreme serum transferrin concentrations, high trisialotransferrin and interfering conditions (haemolysed, lactescent and icteric samples). The method was compared with two routine procedures, the %CDT TIA (Bio-Rad, Hercules, CA, USA) and the CapillarysTM CDT (Sebia, France). A total of 350 clinical sera samples were used for a case-control study. Results: Precision values were better in high CDT and medium CDT pools than in low CDT pools. The serum transferrin concentration had no effect on CDT measurement, except in samples with serum transferrin <1 g/L. Haemolysis was the only interfering situation. The method showed high correlation (r2 > 0.95) with the two other methods (%CDT TIA and CZE %CDT). The global predictive value of the test was >0.90 at 1.9% cut-off. Conclusions: These results demonstrate that the %CDT by the HPLC test is suitable for CDT routine measurement; the results from the high-throughput VariantTM system are well correlated with other methods and are of high diagnostic value.


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