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Alcohol and Alcoholism Vol. 38, No. 2, pp. 115-120, 2003
© 2003 Medical Council on Alcohol

CONTINUOUS ETHANOL ADMINISTRATION INFLUENCES RAT BRAIN 5-HYDROXYTRYPTAMINE SYNTHESIS NON-UNIFORMLY: {alpha}-[14C]METHYL-L-TRYPTOPHAN AUTORADIOGRAPHIC MEASUREMENTS

Fumitaka Yamane1, Yoshihiro Tohyama2 and Mirko Diksic*

Cone Laboratory for Neurosurgical Research, Department of Neurology and Neurosurgery, and Montreal Neurological Institute, McGill University, 3801 University Street, Montreal, Quebec, H3A 2B4 Canada

Received 2 August 2002; first review notified 4 November 2002; accepted 14 November 2002

— Aims: The influence of alcohol on the brain serotonergic system has been studied for several decades with some discordant results. The effects of continuous and constant treatment with ethanol on the rates of serotonin [5-hydroxytryptamine (5-HT)] synthesis in discrete regions of the rat brain were studied. Methods: 5-HT synthesis rates were measured using the {alpha}-[14C]methyl-l-tryptophan autoradiographic method. The rats in the experimental group were treated with 50% ethyl alcohol and those in the control group received distilled water. The fluid was delivered subcutaneously by implanted osmotic mini-pumps for 14 days at the rate of 5 µl/h or 0.12 ml/day (0.06 ml of alcohol per day). Results: Chronic ethanol treatment, as delivered in the present experiment, induced a significant increase in the rate of 5-HT synthesis in descending serotonergic cell bodies (raphe pallidum, raphe obscurus, raphe magnus), nigrostriatal structures, the hippocampus and cortices. No significant changes were observed in the dorsal and median raphe nuclei or pineal body. The results suggest that there may be differences in the regulation of 5-HT synthesis in different brain structures after 14 days of continuous (subcutaneous) injection of 50% alcohol. Conclusions: Chronic ethanol treatments using osmotic mini-pumps induce non-uniform increases in 5-HT synthesis in the rat brain.


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