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© 1998 Medical Council on Alcohol

other

CDTect-RIA AND CDTect-EIA FOR DETERMINATION OF SERUM CARBOHYDRATE-DEFICIENT TRANSFERRIN COMPARED

TORSTEN ARNDT*, JUERGEN KROPF, RAGNHILD BRANDT1, AXEL M. GRESSNER, ROLF HACKLER, MANFRED HEROLD2, JOHANNES VAN PELT3, OLA MÅRTENSSON1, KARIN SALZMANN2 and MATHIEU H. VELMANS3

Klinikum der Philipps-Universitàt Marburg, Abteilung für Klinische Chemie und Zentrallaboratorium BaldingerstraBe, D-35033 Marburg, Germany
1Pharmacia & Upjohn Diagnostics AB, S-75182 Uppsaia. Sweden
2Universitats Klinik für Innere Medizin Hauptlabor, AnichstraBe 35, A-6020 Innsbruck, Austria
3St Maartens Gasthuis, Klinisch Chemisch en Hematologisch Laboratorium PO Box 1926, NL-5900 BX Venlo, The Netherlands

*Author to whom correspondence should be addressed at: bioscientia, Institut für Laboruntersuchungen Ingelheim GmbH, Hamburger Str. 1, D-552l8 Ingelheim. Germany.

Received 16 February 1998; first review notified 24 April 1998; accepted 11 May 1998

CDTect-RIA and CDTect-EIA for determination of serum carbohydrate-deficient transferrin (CDT) by radioimmunoassay and enzyme immunoassay respectively were tested for equality and precision in four European laboratories. For correlational studies, serum samples with CDT concentrations up to 130 U/1 were analysed in accordance with a uniform trial schedule. The regression of CDT values obtained by the two procedures was computed for each laboratory using the method of Passing and Bablok. Slopes and intercepts of the regression functions did not differ significantly from the values 1 or 0, as proved by the corresponding 95% confidence intervals. Precision studies were computed using analysis of variance. For CDT concentrations at the upper reference limit for men, the within-day coefficients of variation (CVs) ranged between 0.7 and 6.4% (median 5.2%) for CDTect-RIA and from 4.3 to 9.2% (median 6.2%) for CDTect-EIA. The corresponding pure between-day CVs were 5.0–18.5% (median 9.8%) and 3.5–14.5% (median 10.9%). The study demonstrates the equality of CDT values obtained by CDTect-RIA and CDTect-EIA. According to this study, the two methods can be used interchangeably without getting fluctuating CDT values, e.g. in longitudinal studies.


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[Abstract] [Full Text] [PDF]


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