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© 1997 Medical Council on Alcohol

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REGULATION OF RAT NEURONAL NITRIC OXIDE SYNTHASE ACTIVITY BY CHRONIC ALCOHOLIZATION

M. NASSILA, F. BEAUGE1 and M. DAOUST*

INSERM U295, Université de Médecine Pharmacie BP 97, 76803 Saint Etienne du Rouvray cédex, France
1Centre de Recherche Pernod Ricard 94015 Créteil, France

*Author to whom correspondence should be addressed

Received 25 June 1996; first review notified 1 September 1996; accepted 3 September 1996

Rat neuronal nitric oxide (NO) synthase (nNOS) activity was measured in frontal cortex, hippocampus, striatum and cerebellum using the assay of [3H]citrulline, following chronic alcoholization. The Km and Vmax values were significantly increased in the frontal cortex and in the striatum, and were not affected in the cerebellum and hippocampus.


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