© 1996 Medical Council on Alcohol
research-article
CARBOHYDRATE-DEFICIENT TRANSFERRIN MEASURED BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND CDTectTM IMMUNOASSAY
Department of Clinical Chemistry, Hospital of Engelholm S-26281 Engelholm, Sweden
1Department of Medical Neurochemistry, Lund University Lund, Sweden
*Author to whom correspondence should be addressed at: Department of Clinical Chemistry. Malmö University Hospital, S-2O5O2 Malmö, Sweden
Received 13 November 1995; first review notified 7 March 1996; accepted 11 March 1996
Carbohydrate-deficient transferrin (CDT) was measured in three populations using anion-exchange chromatography followed by radioimmunoassay (CDTecTM) and a new high-performance liquid chromatography (HPLC) method. The correlation between the methods in 50 consecutive clinical samples was good (r= 0.87). However, in a set of 49 samples with CDT concentrations close to the reference value of the methods, the correlation was low (r= 0.51)- In addition, among controls, no correlation between the methods was found (r= 0.10). Abnormal isoforms of transfemn were noted in 5% of the clinical samples. HPLC determination of CDT offers the advantage of identifying these isoforms of transfemn and thus reducing analytical pitfalls associated with the CDTectTM method.
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