© 1996 Medical Council on Alcohol
research-article
SHOULD TRI-SIALO-TRANSFERRINS BE INCLUDED WHEN CALCULATING CARBOHYDRATE-DEFICIENT TRANSFERRIN FOR DIAGNOSING ELEVATED ALCOHOL INTAKE?
Axis Biocheiracals AS P.O.B. 2123 GrünerlØkka, N-0505 Oslo, Norway
*Author to whom correspondence should be addressed
Received 11 September 1995; first review notified 29 January 1996; accepted 16 February 1996
CDT (carbohydrate-deficient transferrin) has been identified as a specific marker for chronically elevated alcohol consumption. We investigated the sensitivity and accuracy of using relative concentrations of different isotransferrins in serum for diagnosis of chronically elevated alcohol consumption. The different transferrin variants (isoforms) were quantified by HPLC. Including the tri-sialo-transferrin fraction into the definition of %CDT resulted in an increased accuracy in the detection of chronically elevated alcohol intake in a study among 17 heavy drinkers, 25 healthy individuals with moderate alcohol consumption and nine total abstainers. The results also suggest that desialylation of transferrin is a gradually continuing process, rather than one leading to a single end-result separating asialo-, mono- and disialo-transferrins from trisialo-, tetrasialo-, pentasialo- and higher sialo-transferrins.
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