Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (35)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by MAHER, J. J.
Right arrow Articles by GIMÉNEZ, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by MAHER, J. J.
Right arrow Articles by GIMÉNEZ, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1994 Medical Council on Alcohol

research-article

MALONDIALDEHYDE STIMULATES COLLAGEN PRODUCTION BY HEPATIC LIPOCYTES ONLY UPON ACTIVATION IN PRIMARY CULTURE

JACQUELYN J. MAHER, CHRISTINA TZAGARAKIS and AMÉRICA GIMÉNEZ

Liver Core Center and Department of Medicine, University of California, San Francisco General Hospital 1001 Potrero Avenue, San Francisco, CA 94110, U.S.A.

Received 15 August 1993; first review notified 18 February 1994; Lipid aldehydes have been proposed as mediators of hepatic fibrosis in alcoholics. In this study we examined whether hepatic lipocytes, the principal matrix-producing cells in liver, exhibit enhanced collagen synthesis in response to the lipid aldehyde malondialdehyde. Upocytea isolated from normal rat liver and plated in primary culture for 3 days were not affected by malondialdehyde in concentrations ranging from 2 to 200 µM. Cells cultured for 7 days displayed a modest increase in collagen synthesis (137% of control levels) in response to malondialdehyde, but only at a concentration of 200 µM. The malondialdehyde-induced increase in collagen synthesis was paralleled by a rise in type I procollagen mRNA. Subcultured rat fibroblasts at confluent density responded better to malondialdehyde than did 7-day lipocytes. The results indicate that lipocytes respond to the fibrogenic effects of malondialdehyde only after activation in primary culture. This delayed response suggests that lipid aldehydes may enhance, but do not initiate, alcoholic liver fibrosis in vivo.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
I. Garcia-Ruiz, P. de la Torre, T. Diaz, E. Esteban, I. Fernandez, T. Munoz-Yague, and J. A. Solis-Herruzo
Sp1 and Sp3 Transcription Factors Mediate Malondialdehyde-induced Collagen alpha 1(I) Gene Expression in Cultured Hepatic Stellate Cells
J. Biol. Chem., August 16, 2002; 277(34): 30551 - 30558.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.